Coding

Part:BBa_K3645007:Design

Designed by: Huang nan   Group: iGEM20_Peking   (2020-10-26)


dCas9 for ABE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1096
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Contains two mutation and no start codon


Source

From Peking 2019

References